| 朱丽颖,胡家安,高建东.基于生物信息学的八珍汤改善老年肌少症的机制研究[J].老年医学与保健,2026,32(3):424-431 |
| 基于生物信息学的八珍汤改善老年肌少症的机制研究 |
| Research on mechanism of Baizhen decoction based on bioinformatics in improving sarcopenia in the elderly |
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| DOI：10.3969/j.issn.1008-8296.2026.03.018 |
| 中文关键词: 八珍汤 肌少症 免疫炎症 网络药理学 生物信息学 |
| 英文关键词: Bazhen decoction sarcopenia immune-inflammation network pharmacology bioinformatics |
| 基金项目:202340151:上海市卫生健康委员会科研项目 |
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| 中文摘要: |
| 目的 探讨中药八珍汤对老年肌少症的治疗机制.方法 利用公共数据库遴选八珍汤中药目的基因(HRTG)与肌少症差异表达基因(DEG)的重叠基因,进行基因集富集分析、功能富集分析.构建排序前10 基因的蛋白--蛋白相互作用(PPI)网络.绘制排序前3 枢纽基因的受试者操作特征(ROC)曲线.比较老年肌少症组和对照组之间免疫细胞浸润差异.对具有差异的免疫细胞之间、枢纽基因与免疫细胞之间进行相关性分析.结果 研究共获得 105 个八珍汤HRTG,6 594 个肌少症 DEG,39 个 HRTG-DEG.基因本体论富集分析显示,HRTG-DEG 主要富集在氧化应激反应、炎症因子和免疫细胞功能通路中.京都基因和基因组数据库富集分析发现,HRTG-DEG 富集在脂质与动脉粥样硬化、晚期糖基化终末产物及其受体信号通路、炎症因子和免疫细胞功能等通路中.ROC 曲线显示,PPI 网络得到的 8 个枢纽基因中肿瘤坏死因子、白细胞介素-6 和胱天蛋白酶3 对肌少症有预测作用.肌少症中 15 种免疫细胞异常浸润,且这 15 种免疫细胞之间具有相关性(P<0.05).结论 八珍汤可能通过调控免疫炎症改善肌少症. |
| 英文摘要: |
| Objective To investigate the mechanism of Bazhen decoction(BZD)in the treatment of sarcopenia in the elderly.Methods Public databases were used to select overlapping genes between the hub-related target genes(HRTG)of BZD and the differentially expressed genes(DEG)associated with sarcopenia.Gene set enrichment analysis and functional enrichment analysis were performed on these overlapping genes.A protein-protein interaction(PPI)network was constructed for the top 10 genes.Receiver operator characteristic(ROC)curves were plotted for the top 3 hub genes identified from the PPI network.Differences in immune cell infiltration were compared between the elderly sarcopenia group and the control group.Correlation analysis was conducted among the differential infiltrated immune cells,and between the hub genes and immune cells.Results A total of 105 HRTGs of BZD,6 594 DEGs of sarcopenia,and 39 HRTG-DEGs were obtained.Gene ontology enrichment analysis showed that the HRTG-DEGs were primarily enriched in pathways related to oxidative stress response,inflammatory factors,and immune cell function.The Kyoto Gene and Genome Database enrichment analysis revealed that HRTG-DEGs were enriched in pathways such as lipid and atherosclerosis,advanced glycation end products and their receptor signaling pathways,inflammatory factors,and immune cell functions.ROC curve analysis indicated that among the 8 hub genes from the PPI network,tumor necrosis factor,interleukin-6,and caspase-3 had predictive value for sarcopenia.There were 15 abnormal infiltration of immune cells in sarcopenia,and these 15 immune cells were correlated with each other(P<0.05).Conclusion BZD may improve sarcopenia by regulating immune-inflammatory responses. |
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